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General description
High affinity immunoglobulin epsilon receptor subunit gamma (UniProt: P30273; also known as Fc receptor gamma-chain, FcRgamma, Fc-epsilon RI-gamma, IgE Fc receptor subunit gamma, FceRI gamma) is encoded by the FCER1G gene (Gene ID: 2207) in human. FceRI g is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a short extracellular domain (aa 19-23), a transmembrane domain (aa 24-44), and a cytoplasmic domain (aa 45-86). FceRI g serves as an adapter protein that contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. The g-subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. It associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. As a component of the high-affinity immunoglobulin E (IgE) receptor, it is shown to mediate allergic inflammatory signaling in mast cells and as a constitutive component of interleukin-3 receptor complex, it selectively mediates interleukin 4/IL4 production by basophils and primes T-cells toward effector T-helper 2 subset. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human High affinity immunoglobulin epsilon receptor subunit gamma (FceRI g ).
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse Spleen tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FceRI g-subunit subunit in Mouse Spleen tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected FceRI g-subunit in RBL and U-937 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FceRI g-subunit subunit in U-87 MG cell line.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FceRI g-subunit in human spleen tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound FceRI g-subunit peptide with a KD of 3.0 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected FcERI y subunit in Human peripheral blood mononuclear cells (PBMC).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-FceRI g-subunit, clone 1B6 ZooMAb®, Cat. No. ZRB1259, is a recombinant Rabbit monoclonal antibody that specifically targets FceRI g-subunit & is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, & WB.
Biochem/physiol Actions
Clone 1B6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects High affinity immunoglobulin epsilon receptor subunit gamma (FceRI g ). It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B6, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt ~9.67 kDa,observed mol wt ~10 kDa |
| species reactivity | rat, human, mouse |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P30273 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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