- 商品介绍
- 规格参数
- 包装参数
General description
The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.
The ryanodine receptor (RyR) is the channel responsible for the release of Ca2+ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca2+ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
Immunogen
partially purified chicken pectoral muscle ryanodine receptors (RyR)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.
Physical form
Purified IgG1 antibody containing 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 34C, monoclonal |
| mol wt | antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms) |
| species reactivity | human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep |
| technique(s) | direct immunofluorescence: suitable,immunohistochemistry (frozen sections): 1:1,000,immunoprecipitation (IP): suitable,western blot: 1:5,000 |
| isotype | IgG1 |
| UniProt accession no. | P21817 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... RYR1(6261), RYR2(6262) mouse ... Ryr1(20190), Ryr2(20191) rat ... Ryr1(114207), Ryr2(84025) |
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