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General description
Interferon regulatory factor 5 (UniProt: Q13568; also known as IRF-5) is encoded by the IRF5 gene (Gene ID: 3663) in human. The interferon regulatory factors (IRFs) are a family of nine transcription factors that regulate pathogen-induced innate and acquired immune responses. In unstimulated cells they reside in the cytoplasm. IRF-5 plays a critical role in innate immunity by activating expression of type I interferons (IFN- and β) and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8, and TLR9. It regulates the transcription of IFN genes (IFNA and IFNB) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It is shown to be a key transcription factor regulating the interferon response during SARS-CoV-2 infection. It is maintained as a monomer in an autoinhibited state and undergoes homodimerization and activation following its phosphorylation on serine 451 and 462 in the C-terminal autoinhibitory region. It has been reported that phosphorylation of serine 451 relieves the autoinhibitory conformation, and phosphorylation of serine 462 stabilizes the IRF-5 homodimer. In its phosphorylation and activation scheme, the innate adapter protein TASL (TLR adaptor interacting with SLC15A4 on the lysosome) recruits IRF-5, which is then phosphorylated by IKK-β at serine 462 that allows IRF-5 dissociation from the adapter protein followed by its dimerization and nuclear translocation. Mutations in IRF5 gene have been linked to systemic lupus erythematosus 10 (SLEB10), rheumatoid arthritis, and irritable bowel syndrome. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Thompson, CD., et al. (2018). Front. Immunol. 9; 2622; Lopez-Pelaez, M., et al. (2014). Proc. Natl Acad. Sci. USA. 111(49); 17432-17437; Foreman, HCC., et al. (2012). PLoS One. 7(3); e33098).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Interferon regulatory factor 5 (IRF-5).
Application
Quality Control Testing
Evaluated by Western Blotting in LNCap cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IRF-5 in LNCap cell nuclear extract.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IRF-5 in THP-1 and NIH3T3 cell nuclear extracts.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected IRF-5 in one million THP-1 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IRF-5 in THP-1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IRF-5 in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound IRF-5 peptide with a KD of 4.9 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in LNCap cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IRF-5 in LNCap cell nuclear extract.
Biochem/physiol Actions
Clone IL11 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Interferon regulatory factor 5 (IRF-5). It targets an epitope within 19 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1L11, recombinant monoclonal |
| description | 1L11 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 56.04 kDa,observed mol wt ~56 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001092097 |
| UniProt accession no. | Q13568 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IRF5(3663) |
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