5R-PLEX beads，5ML，MBD6009-5ML，Sigma

General description

Solid Phase Reversible Immobilization (SPRI) beads are magnetic beads that can be utilized for DNA purification and size selection. The 5R-PLEX beads are SPRI beads designed to easily separate the desired PCR amplicon from nucleotides, adapters, primers, and primer dimers by efficient purification and size selection. The magnetic beads are provided in a ready to use suspension that enables fast and efficient application even in high throughput applications with automated liquid handling. The beads are formulated with NaCl and high purity PEG as the crowding agent.

Application

• NGS library preparation, including 16S and 18S
• Approved to use in ultrasensitive 5R-Plex (SMURF) library preparation(2,3,4) for degraded samples
• PCR reaction clean up
• Amplicon size selection
• DNA purification and manipulation (replacing elution buffer, concentrating the sample)

Features and Benefits

• Ready to use
• Compatible with automated liquid handling
• Easy size selection by changing the volumetric ratio of beads to samples