CRYO-DMSO-F，，C9252-100ML，Sigma

General description

Cryo-DMSO-F is a fully defined cryopreservation medium that is serum-free, protein-free, animal origin-free, and DMSO-free. It is specifically designed for preparing and preserving cells in ultra-low temperature environments ranging from -80 to -196°C, ensuring a safe and protective environment during freezing, storage, and thawing while also expanding storage temperature versatility. The special formulation in CRYO-DMSO-F significantly reduces freezing-induced apoptosis and necrosis, enhancing post-thaw cell viability and function. This medium is recommended for the preservation of CAR-T, NK, and macrophage cells.


Disclaimer: This product is labeled as "DMSO-free." However, it may contain trace amounts of DMSO from a carrier solvent. This concentration is not expected to have any cryopreservative effect and is unlikely to exert toxic effects on cells. The term "DMSO-free" is intended to convey that the product is primarily free of DMSO.

Application

Cryo-DMSO-F is a chemically defined DMSO-free cryopreservation medium. Despite its widespread use as a cryoprotectant in cell freezing, DMSO can be detrimental to cells due to its toxicity. Some cell types are more affected than others, but prolonged exposure to DMSO, particularly after thawing, can impact post-thaw cell viability as well as the overall functionality of the cells themselves. Using a DMSO-free solution can prevent this potential impact.

Features and Benefits

Benefits:

• Provides effective cryopreservation to protect and preserve cell viability during storage in both liquid nitrogen and ultralow temperatures (-80 C).
• Ready-to-use solution simplifies the cryopreservation process, reducing preparation time and minimizing potential errors.
• Minimized contamination risk through sterile filtration, enhancing safety and reliability for sensitive cell cultures.

• DMSO-free formulation ensures better viability and phenotypic maintenance for sensitive cell types like stem cells.
• Designed for ease of use with complete formulation, eliminating the need for additional media preparation steps.