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General description
Glucuronoxylomannan (GXM) is the principal capsular polysaccharide of Cryptococcus neoformans, a pathogenic fungus causing cryptococcosis, particularly in immunocompromised individuals. Structurally, GXM comprises an -(1→3)-linked mannose backbone substituted with xylose and glucuronic acid residues, and it is O-acetylated at specific mannose units, contributing to its heterogeneity. Synthesized intracellularly, GXM is transported via vesicles to the extracellular space, where it integrates into the fungal capsule and interacts with cell wall components such as glucans and chitin. GXM is critical for fungal virulence, providing protection against phagocytosis, modulating cytokine secretion, and interfering with leukocyte migration. It binds to host receptors, including Toll-like receptors (TLR2, TLR4), CD14, and CD18, facilitating immune evasion. GXM exhibits potent immunosuppressive properties by inhibiting pro-inflammatory cytokines (e.g., IL-1β, TNF- ) while promoting anti-inflammatory cytokines like IL-10. This immunomodulation contributes to disease progression, including cryptococcal meningitis. Additionally, GXM has shown promise as an anti-inflammatory agent in experimental models of septic arthritis, reducing inflammation and cytokine levels. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Monari, C., t al., (2006), FEMS Yeast Research, 6(4), 537 542; Chang, Z.L., et al., (2006), Infect Immun, 74(1), 144-151)
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
Cryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.
Application
Quality Control Testing
Immunohistochemistry (Paraffin) Analysis on mouse spleen tissue sections.
Immunohistochemistry (IHC) Paraffin: A 1:100 dilution from a representative lot detected GXM in mouse spleen tissue sections.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GXM in C. neoformans-infected mouse liver, brain, and lung tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 18B7 is a mouse monoclonal antibody that binds to Glucuronoxylomannan (GXM) from Cryptococcus neoformans.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 18B7, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| purified by | using protein G |
| species reactivity | yeast |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunohistochemistry: suitable |
| isotype | IgG1κ |
| epitope sequence | Unknown |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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