ppTG21 trifluoroacetate salt，1MG，SML3052-1MG，Sigma

Biochem/physiol Actions

Endosomolytic agent that facilitates cell type-specific gene editing by enabling Cas9 RNP endosome escape following receptor-mediated endocytosis.
ppTG21 is an endosomolytic agent (pI 7.7, charge +1.3 @ pH 7.4) that facilitates cell type-specific gene editing by enabling endosome escape of Cas9 ribonucleoprotein (RNP) complex following its uptake via receptor-mediated endocytosis. Following liver-specific asialoglycoprotein receptor (ASGPr)-mediated delivery of ASGPr ligand (ASGPrL)-harboring & SV40 nuclear localization sequence (NLS)-bearing S. pyogenes Cas9 (Cas9-2lig-1NLS) RNP with EMX1-targeting sgRNA, target gene editing occurs only upon coincubation of the RNP in the presence of 30 molar equiv of ppTG21 (ave 4.8% indels in HepG2 cells vs. 0.2% indels without ppTG21).