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General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Natural resistance-associated macrophage protein 2 (UniProt: P49281; also known as NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1, DMT-1, Solute carrier family 11 member 2) is encoded by the SLC11A2 (also known as DCT1, DMT1, NRAMP2) gene (Gene ID: 4891) in human. It is a proton-coupled Fe2+ transporter that is essential for iron uptake in enterocytes and for transferrin-associated endosomal iron transport in many other cell types. SLC11A2 can transport manganese, cobalt, cadmium, nickel, and vanadium. Structurally, SLC11A2 is a multi-pass membrane protein characterized by multiple transmembrane domains that allow it to function as a symporter, coupling the transport of protons with divalent cations such as Fe²⁺. It is ubiquitously expressed and also found in erythroid progenitors. Its expression is particularly prominent in the duodenum, where it is essential for the absorption of dietary iron, and it is also found in the brain, liver, and various immune cells. SLC11A2 is localized to the plasma membrane and endosomal compartments, where it mediates the uptake of iron in a pH-dependent manner. Five isoforms of SLC11A2 have been described that are produced by alternative splicing. have been described that are produced by alternative splicing. Mutations in the SLC11A2 gene have been implicated in several diseases, most notably hypochromic microcytic anemia and iron overload disorders, as these mutations can disrupt normal iron uptake and lead to abnormal iron accumulation in tissues. Additionally, SLC11A2 dysfuntion has been associated with the pathogenesis of neurodegenerative diseases, such as Alzheimer′s disease, due to its role in regulating metal ion homeostasis that influences amyloid beta aggregation. Recent study highlights pivotal role of SLC11A1 and SLC11A2 in iron metabolism, cellular homeostasis, processes linked to oncogenesis and, suggest their potential as biomarkers and therapeutic targets. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Banerjee, R., et al. (2025). Cell Commun. Signal. 23; 332).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal cytoplasmic domain of human SLC11A2.
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC11A2 in HepG2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SLC11A2 in HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC11A2 in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound SLC11A2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 3F18 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects SLC11A2. It targets an epitope within 22 amino acids from the N-terminal cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3F18, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 62.27 kDa,observed mol wt ~65-80 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal cytoplasmic domain |
| Protein ID accession no. | NP_001167597 |
| UniProt accession no. | P49281 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... SLC11A2(4891) |
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