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General description
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (UniProt: Q15274; also known as EC:2.4.2.19, Quinolinate phosphoribosyltransferase [decarboxylating], QAPRTase, QPRTase) is encoded by the QPRT gene (Gene ID: 23475) in human. QPRTase is a hexameric enzyme formed by three homodimers. It is involved in de novo synthesis of NAD using quinolinic acid (QA) as a precursor that is produced via the degradation of tryptophan in kynurenine pathway. In mammals QA is shown to be an excitotoxic compound that can lead to neurodegeneration via over activation of NMDA receptors. The only known known pathway for QA elimination is via decarboxylation and conjugation with phosphoribosylpyrophosphate to form nicotinamide mononucleotide (NaMN) that is catalyzed by QPRTase. In brain, QPRTase is shown to be preferentially localized in glial cells and in neurons of the cerebral ventricles. Its levels in liver are shown to be several-folds higher than in brain. QPRTase has three QA binding sites (aa 138-139; 160-161; and 248-250) and the region consisting of amino acids 8-12 is important for hexamer formation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fukuoka, SI., et al. (1998). Biochim. Biophys. Acta. 1395(2); 192-201; Youn, HS., et al. (2016). Sci. Rep. 6; 19681).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Immunogen
His-tagged full-length recombinant human Quinolinate phosphoribosyltransferase (QPRTase).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected QPRTase in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected QPRTase in lysates from human brain, and C6, and HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound recombinant QPRTase with a KD of 1.3 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected QPRTase in human kidney tissue sections.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected QPRTase in one million HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected QPRTase in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-QPRTase, clone 1G22 ZooMAb®, Cat. No. ZRB1110, is a recombinant Rabbit monoclonal antibody that specifically targets QPRTase and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Biochem/physiol Actions
Clone 1G22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Quinolinate phosphoribosyltransferase (QPRTase).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1G22, monoclonal,recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt ~30.85 kDa,observed mol wt ~35 kDa |
| species reactivity | human, rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q15274 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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