- 商品介绍
- 规格参数
- 包装参数
General description
Muscarinic acetylcholine receptor M4 (UniProt: also known as Muscarinic M4 receptor) is encoded by the CHRM4 gene (Gene ID: 1132) in human. Acetylcholine receptors are classified as muscarinic or nicotinic. Muscarinic receptors are found in neuroeffector junctions and are coupled to G-proteins. On the other hand, nicotinic receptors are found in the autonomic ganglia and neuromuscular junctions. Muscarinic receptors are G-protein-coupled receptors that are involved in the transduction of cholinergic signals in the central nervous system, autonomic ganglia, smooth muscles, and other parasympathetic end organs. Five different subtypes of muscarinic receptors have been described (M1-M5). The M1, M3, and M5 receptors are excitatory receptors, whereas M2 and M4 receptors inhibit neuronal firing by decreasing the intracellular concentration of cAMP and activating G-protein coupled inward rectifying potassium channels. M1, M3, and M5 receptors are coupled to Gq/11 protein, while M2 and M4 pre
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
GST-tagged recombinant fragment corresponding to the third intracellular loop of human muscarinic acetylcholine receptor M4 (CHRM4).
Application
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Muscarinic acetylcholine receptor M4 (CHRM4) in SHSY5Y cell lysate.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound full-length recombinant Human CHRM4 protein with a KD of 5.1 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHRM4 in SH-SY5Y cells.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected full-length recombinant Human CHRM4.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 18C7.2 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Muscarinic acetylcholine receptor M4 (CHRM4). It targets an epitope within the third intracellular loop.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 18C7.2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 53.05 kDa,observed mol wt ~56 kDa |
| purified by | using protein G |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| epitope sequence | Cytoplasmic domain |
| Protein ID accession no. | NM_000741.2 |
| UniProt accession no. | P08173 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CHRM4(1132) |
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