SYBR® Green Extract-N-Amp™组织PCR试剂盒，1KIT，XNATRG-1KT，Sigma

General description

The SYBR^® Green Extract-N-Amp^™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Application

SYBR^® Green Extract-N-Amp^™ tissue PCR kit has been used for DNA extraction and genotyping.

Biochem/physiol Actions

DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp^™ SYBR^® Green PCR ReadyMix^™ and user-provided PCR primers.

Features and Benefits

• Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
• Fast – tissue to qPCR in 15 minutes
• Convenient – no long enzymatic digestions and no column purifications
• Simple – rapid, easy-to-follow protocol
• Sensitive – specially formulated HOTSTART^® SYBR^® Green PCR ReadyMix^™ for highly specific PCR amplification and quantitation of genomic DNA
• Safe – no organic extraction with hazardous chemicals

Other Notes

更多信息，请见www.sigma-aldrich.com/extract-n-amp。
试剂盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix^™。Extract-N-Amp SYBR Green PCR ReadyMix为2X反应混合物，其中含有 SYBR Green、缓冲液、盐、dNTP、Taq聚合酶和 JumpStart^™ Taq 抗体。此试剂盒专为结合提取试剂使用进行了优化，其中含有适用于热启动PCR、可增强特异性的 JumpStart Taq抗体，以及可用作实时定量PCR的非特异性报告标志物的SYBR Green I。

Legal Information

本产品的使用受到如下一项或多项美国**及其对应的境外**声明保护：5,994,056和6,171,785。购买本产品，即相当于依照境外的**声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。其他**声明下的任何权利（如美国**号6,814,934号下的设备和系统**声明）、进行任何**方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche**许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。

依照美国**号5,338,671和5,587,287和其他地区对应的**，许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries