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General description
Prolyl 4-hydroxylase subunit alpha-1 (UniProt: P13674; also known as EC:1.14.11.2, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1) is encoded by the P4HA1 (also known as P4HA) gene (Gene ID: 5033) in human. Prolyl 4-hydroxylase is a key enzyme in collagen synthesis and is composed of two identical a subunits and two b subunits. In collagen and related proteins, prolyl 4-hydroxylase is reported to catalyze the formation of 4-hydroxyproline, which is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Three P4HA isoforms (P4HA1-3) have been identified in mammalian cells and P4HA1 is the major isoform described in most cell types and tissues and contributes to the majority of the prolyl 4-hydroxylase activity. It is localized in endoplasmic reticulum lumen and catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Depletion of P4HA1 in melanoma cells is reported to reduce cell adhesion, invasion, and viability in vitro. In melanoma xenograft models, P4HA1 knockdown reduces melanoma tumor invasion and the deposition of collagens, particularly type IV collagen, in the interstitial extracellular matrix and in the basement membranes of tumor blood vessels. This can lead to vessel wall rupture and hemorrhages. Significantly higher levels of P4HA1 are reported in subjects with lung adenocarcinomas and relate to worse overall survival. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, H., et al. (2020). Biomed. Res. Int. 2020, Article ID 8024138; Eriksson, J., et al. (2020). Mol. Oncol. 14(4); 742-762).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the internal region of human Prolyl 4-hydroxylase subunit alpha-1 (P4HA1)
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected P4HA1 in HEK293 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected P4HA1 in U2OS cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected P4HA1 in U2OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected P4HA1 in human placenta tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound P4HA1 with a KD of 9.1 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1C14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Prolyl 4-hydroxylase subunit alpha-1 (P4HA1), It targets an epitope within 25 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C14, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 61.05 kDa,observed mol wt ~59 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_001017962 |
| UniProt accession no. | P13674 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... P4HA1(5033) |
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