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General description
Leucine-rich repeat flightless-interacting protein 2 (UniProt: Q9Y608; also known as LRR FLII-interacting protein 2, LRRFIP2) is encoded by the LRRFIP2 gene (Gene ID: 9209) in human. LRRFIP2 is a myeloid differentiation factor 88 (MYD88)-interacting protein with a positive regulatory function in toll-like receptor signaling. It is widely expressed with highest levels observed in heart and skeletal muscle. Five isoforms of LRRFIP2 have been described that are produced by alternative splicing. Isoform 1 is long isoform and other isoforms are considered as short isoforms. It interacts with Dvl3 to activate the canonical Wnt signaling pathway and increases β-catenin/ LEF/TCF-dependent transcriptional activity. Its Dvl3-binding region is localized in amino acids 1-370. Both the amino and carboxyl termini of LRRFIP2 are essential for its function. The amino terminus is responsible for interaction with the central region of Dvl3, which contains the PDZ and DEP domains. Mutant form of LRRFIP2 that contains only the amino terminal acts as a dominant negative form. In resting cells, it shows weak interaction with MYD88. However, following LPS-stimulation, the interaction with MYD is rapidly increased and it returns to basal levels after about six hours of stimulation. Serine 190 and 202 of LRRFIP2 are phosphorylated in response to LPS stimulation. Its interaction with MYD is regulated by LPS-induced phosphorylation of serine 202. LRRFIP2 is also reported to negatively regulate NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition. It binds to NLRP3 via its N-terminal upon NLRP3 inflammasome activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jin, J., et al. (2013). Nat. Commun. 4; Article 2075; Dai, P., et al. (2009). J. Immunol. 182(6); 3450-3460; Liu, J., et al. (2005). Proc. Natl. Acad. Sci. USA. 102(6); 1927-1932).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human Leucine-rich repeat flightless-interacting protein 2 (LRRFIP2).
Application
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRRFIP2 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRRFIP2 in lysate from NIH3T3 cell lysates and recombinant fragment of Human LRRFIP2.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRRFIP2 in A431 cells.
Affinity Binding Assay: A representative lot of this antibody bound LRRFIP2 peptide with a KD of 5.4 x 10-6 in an affinity binding assay.
Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected recombinant fragment of Human LRRFIP2 in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1B22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Leucine-rich repeat flightless-interacting protein 2 (LRRFIP2). It targets an epitope within 24 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B22, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 82.17 kDa,observed mol wt ~79 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_006300 |
| UniProt accession no. | Q9Y608 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... LRRFIP2(9209) |
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