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- 规格参数
- 包装参数
General description
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of serine 141 (serine 144 for PAK1 and serine 139 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1 (46% homologous), PAK2, and PAK3 (46% homologous). Other species have not been tested.
Immunogen
synthetic phosphopeptide derived from the region of human PAK2 that contains serine 141.
Application
A working dilution of 1:1000 is recommended for immunoblotting detection in Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.
Biochem/physiol Actions
The antibody is highly selective for the phosphorylated form of PAK and can be used to detect an active form of PAK1, as well as PAK2 and PAK3.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| species reactivity | human |
| technique(s) | western blot: 1:1,000 using Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation |
| UniProt accession no. | Q13153 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer141) |
| Gene Information | human ... PAK1(5058) |
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