Anti-POLR2F antibody produced in rabbit，100UL，HPA000827-100UL，Sigma

Immunogen

DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

DNA-directed RNA polymerases I, II, and III subunit RPABC2 is a protein encoded by the POLR2F gene in humans. The gene is one of the common subunits of all eukaryotic RNA polymerases and is indispensable for the enzyme function. N-terminal acidic region of the gene subunit has phosphorylation-coupled regulatory functions. It is found to be up-regulated in colorectal carcinomas and exhibited elevated levels in carcinomas compared to normal tissue. POLR2F may have a possible role in colorectal cancer.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST73387

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany