Anti-HIS Magnetic Beads，1ML，SAE0222-1ML，Sigma

General description

The Anti-HIS Magnetic Beads (SAE0222) are specially designed for the purification of HIS-tagged proteins expressed in bacterial and mammalian cell lysates. These beads leverage a high-affinity monoclonal anti-HIS antibody, conjugated to magnetic beads, allowing for efficient isolation of proteins of interest. The product is provided as a suspension containing 50% beads in a phosphate-buffered saline (PBS) solution, making it ideal for various laboratory applications. With a binding capacity ranging from 600 to 5000 µg of HIS-tagged fusion protein per ml of settled resin, these beads are a reliable choice for researchers looking to streamline their protein purification processes.

Application

Anti-HIS Magnetic Beads are primarily used for:

• Purification of HIS-tagged Proteins: Ideal for isolating proteins from bacterial and mammalian cell lysates that have been genetically engineered to express HIS tags.
• Immunoprecipitation and Immunoaffinity Purification: The beads can effectively capture HIS-tagged proteins for further analysis, including SDS-PAGE and Western blotting.
• Protein Characterization: Useful for studying protein topology, identifying new proteins, and characterizing protein complexes, especially in cases where specific antibodies are not available.

Features and Benefits

• High Binding Capacity: Capable of binding between 600 to 5000 µg of HIS-tagged proteins, ensuring maximum yield.
• Versatile Use: Suitable for both native and denatured forms of HIS-tagged proteins, allowing flexibility in experimental designs.
• Efficient Isolation: The magnetic property allows for easy separation of beads from the solution, saving time and enhancing workflow efficiency.
• Preserved Protein Activity: The HIS tag does not interfere with the biological activity or solubility of the target protein, ensuring that functional studies remain valid.

Preparation Note

To maintain optimal performance, store the Anti-HIS Magnetic Beads at a temperature between 2°C to 8°C. Avoid freezing to preserve the integrity of the beads. For continuous use, it is recommended to mix the beads thoroughly before use by gentle vortexing or inversion. The product is stable for extended periods under recommended storage conditions, ensuring reliability for ongoing research applications.