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General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Oxidized low-density lipoprotein receptor 1 (UniProt: P78380; also known as Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1 (LOX-1; Lectin-like oxLDL receptor 1; hLOX-1), Lectin-type oxidized LDL receptor 1, Oxidized low-density lipoprotein receptor 1, soluble form) is encoded by the OLR1 (also known as CLEC8A, LOX1) gene (Gene ID: 4973) in human. Oxidized low-density lipoprotein receptor 1 (OLR1), also known as LOX-1, is a transmembrane protein belonging to the C-type lectin superfamily. Structurally, it consists of a short intracellular domain, a transmembrane region, and an extracellular lectin-like domain that facilitates binding to modified lipoproteins, such as oxidized LDL (oxLDL). This receptor plays a critical role in vascular biology by mediating the recognition, internalization, and degradation of oxLDL, which is central to its function as a scavenger receptor. OLR1 is localized to the plasma membrane, nucleoplasm, and vesicles, indicating its dynamic role in cellular processes. OLR1 is involved in inflammation, oxidative stress, and apoptosis. It regulates pathways such as nuclear factor- B (NF- B) signaling, which drives the expression of pro-inflammatory cytokines and adhesion molecules. OLR1 expression is typically low under physiological conditions but is upregulated in response to atherosclerotic stimuli, including tumor necrosis factor-alpha (TNF- ), shear stress, and oxLDL exposure. This upregulation enhances endothelial dysfunction and promotes atherogenesis. OLR1 also plays roles in other pathological contexts, including ischemic stroke, diabetes, and cancer, where its activity contributes to oxidative stress and inflammation. OLR1 interacts with various proteins and signaling pathways, including those involved in lipid metabolism, immune cell activation, and apoptosis. Its expression is regulated by cyclic AMP signaling and is influenced by inflammatory mediators. Mutations in the OLR1 gene have been associated with increased susceptibility to myocardial infarction and other cardiovascular diseases. Soluble OLR1 (sOLR1), which is derived from proteolytic cleavage of the membrane-bound receptor, serves as a biomarker for vascular inflammation and atherosclerosis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human OLR1.
Application
Quality Control Testing
Evaluated by Western Blotting in RAW 264.7 cell lysate.
Western Blotting Analysis: 1:1,000 dilution of this antibody detected OLR1 in RAW 264.7 cell lysate.
Tested Applications
Western Blotting Analysis: 1:1,000 dilution from a representative lot detected OLR1 in human, mouse, and rat kidney tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected OLR1 in human, rat, and mouse kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound with OLR1 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2E2 is a ZooMAb® rabbit recombinant monoclonal antibody that detects OLR1. It targets an epitope within 16 amino acids from the extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2E2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 30.96 kDa,observed mol wt ~52 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_002534.1 |
| UniProt accession no. | P78380 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... OLR1(4973) |
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