Ten Eleven Translocase，25UG，S9797-25UG，Sigma

销售价： ¥ 6944.11 / 件

在线询价

优惠：请询价

订货号： 0BQ4338

品牌型号： Sigma S9797-25UG

货期：询货期

最小订货量： 1件

+ -

加入购物车

联系我们

400-920-3909

微信扫一扫，手机采购

精选商品

C-fos, proto oncogene human，5UG，SRP2087-5UG，Sigma

销售价： ¥ 4589.25

加入购物车

Anti-CX3CL1 Antibody, clone 3O6 ZooMAb® Rabbit Monoclonal，，ZRB3088-4X25UL，Sigma

销售价： ¥ 3967.95

加入购物车

PCR核苷酸混合物，200UL，11581295001，Roche

销售价： ¥ 1552.47

加入购物车

Monoclonal Anti-ACTB antibody produced in mouse，100UL，AMAB91241-100UL，Sigma

销售价： ¥ 4608.39

加入购物车

GenElute™-E Single Spin Blood DNA 96 Kit，8EACH，EC196-8EA，Sigma

销售价： ¥ 24537.45

加入购物车

MCF10A CELLS GSK3B (-/-)，1SET，CLLS1044-1SET，Sigma

销售价： ¥ 44148.85

加入购物车

商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

This product consists of the catalytic domain (CD) of mTET1 (amino acids 1366-2039) and includes an N-terminal polyhistidine tag. It has a calculated molecular mass of 75.8 kDa. This protein is produced in mammalian cells (HEK 293), without the use of serum. The mammalian cells expression system allows proper post-translational modifications and folding, and often supports higher specific activity of the protein.

Biochem/physiol Actions

Mouse ten eleven translocase 1 (mTET1) is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and plays a key role in active DNA demethylation. mTET1 also causes the sequential oxidation of 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). The full structure of TET1 includes a double-stranded β-helix (DSBH) domain, a cysteine-rich domain, and a CXXC domain. The dioxygenase activity of DSBH is dependent on Fe2+ and 2-oxoglutarate.
mTET1-mediated cytosine methylation on C5 is a mammalian genome epigenetic modification, which is involved in transcriptional regulation.
TET1 is utilized in TET-assisted bisulfite sequencing (TAB-seq), a method for the identification of 5hmC at single-base resolution, and the determination of its abundance at each modification site. This method is an improvement of the traditional bisulfite sequencing, which has been widely used to detect 5mC at single-base resolution, but cannot differentiate 5mC from 5hmC, as both resist deamination during the treatment of DNA with sodium bisulfite. The TAB-seq method uses mTET1 to selectively convert 5mC to 5caC in two steps: protection of 5hmC through glucosylation, and mTET1-mediated oxidation of 5mC to 5caC.