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General description
Nectin-2 (UniProt: Q92692; also known as Herpes virus entry mediator B, Herpesvirus entry mediator B, HveB, Nectin cell adhesion molecule 2, Poliovirus receptor-related protein 2, CD112) is encoded by the NECTIN2 (also known as HVEB, PRR2, PVRL2) gene (Gene ID: 5819) in human. Nectin-2 is a single-pass type I membrane protein that displays ubiquitous expression. It is a Ca2+-independent cell-cell adhesion molecule that regulates cell adhesion between epithelial cells through the formation of transdimers between adjacent cells. Nectin-2-mediated cell adhesion is shown to induce the formation of E-cadherin-based adherens junctions followed by the formation of claudin based tight junctions. Nectin-2 also serves as a modulator of T-cell signaling and it can be either a co-stimulator of T-cell function, or a co-inhibitor depending on the receptor it binds to. Upon binding to CD226, it stimulates T-cell proliferation and cytokine production, including that of IL-2, IL-5, IL10, IL-13, and Interferon-g. Its interaction with PVRIG, inhibits T-cell proliferation. These interactions are shown to be competitive in nature. Over-expression of Nectin-2 has been reported in breast and ovarian cancer tissues and in several cancer cell lines. It is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-360), a transmembrane domain (aa 361-381), and a cytoplasmic domain (aa 382-538). Nectin-2 has one Ig-Like V-type domain (aa 32-156) and two Ig-like C2 type domains (aa 162-256 and 261-345). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oshima, T., et al. (2013). Mol. Cancer. 12; 60).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal extracellular domain of human Nectin-2.
Application
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nectin-2/CD112 in MCF-7 cell lysate.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nectin-2 in MCF-7 cells.Affinity Binding Assay: A representative lot of this antibody bound Nectin-2 peptide with a KD of 3.9 x 10-9 in an affinity binding assay.Flow Cytometry Analysis: 1 μg from a representative lot detected Nectin-2 in one million MCF-7 cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Nectin-2 in human testis tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Nectin-2/CD112, clone 2H6 ZooMAb, Cat. No. ZRB1029, is a Rabbit monoclonal antibody that specifically targets Nectin-2/CD112 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Biochem/physiol Actions
Clone 2H6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Nectin-2. It targets an epitope within 24 amino acids from the N-terminal, extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| clone | 2H6, recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 57.74 kDa,observed mol wt ~58 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q92692 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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