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General description
Serotransferrin (UniProt: P02787; also known as Transferrin, Beta-1 metal-binding globulin, Siderophilin) is encoded by the TF (also known as PRO1400) gene (Gene ID: in human. Transferrin is a monomeric glycoprotein that is expressed mainly in the liver and is secreted in plasma. Its functions are mediated via binding to transferrin receptor. It serves as an iron binding and transport protein that can bind two Fe3+ ions in association with the binding of an anion, usually a bicarbonate. It transports iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form. The mature form consists of alpha helices and beta sheets that form two domains. The N and C- terminal sequences are represented as globular N- and C- lobes that are connected with a short peptide. The carbohydrate moiety is attached to the C-lobe. Each lobe is further subdivided into two sub-domains- N1 and N2 for the N- lobe; and C1 and C2 for the C-lobe. Higher levels of transferrin are reported under conditions of anemia and during pregnancy. Mutations in TF gene are linked to Atransferrnemia that is characterized by abnormal synthesis of transferrin leading to iron overload and microcytic hypochromic anemia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
GST/His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal half of the mature human Transferrin.
Application
Anti-Transferrin, clone 2B8 ZooMAb, Cat. No. ZRB1225, is a highly specific rabbit recombinant monoclonal antibody that targets Transferrin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Transferrin in human kidney tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Transferrin in NIH 3T3 and HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Transferrin in human liver tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Biochem/physiol Actions
Clone 2B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine Transferrin. It targets an epitope within the N-terminal region of mature transferrin.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2B8, recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 77.06 kDa,observed mol wt ~77 kDa |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P02787 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TF(7018) |
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