Lenti CRISPR Universal Non-Target Control#2 Plasmid DNA (LV04 vector),1EA,CRISPR19-1EA,Sigma

销售价: ¥ 2198.30 / 件
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订货号 0BQ8952
品牌型号 Sigma CRISPR19-1EA
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The Lenti CRISPR Non-Target Control#2 (Lv04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.


Application

Functional Genomics/Screening/Target Validation


Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).


Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement


技术参数 Specifications
formliquid
Quality Segment200
packagingvial of 50 μL
concentration20 ng/μL in TE buffer; DNA (1μg of plasmid DNA )
application(s)CRISPR
shipped indry ice
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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