PDE1B Active human,10UG,SRP0258-10UG,Sigma

销售价: ¥ 6836.39 / 件
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订货号 0BR1058
品牌型号 Sigma SRP0258-10UG
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.


Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.


Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.


Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.


Other Notes

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C


技术参数 Specifications
biological sourcehuman
recombinantexpressed in baculovirus infected insect cells
assay≥70% (SDS-PAGE)
formaqueous solution
specific activity≥2000 units/μg
mol wt 87 kDa
packagingpkg of 10 μg
concentration>0.02 mg/mL
NCBI accession no.NM_000924
UniProt accession no.Q01064
shipped indry ice
storage temp.−70°C
Gene Informationhuman ... PDE1B(5153)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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