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General description
IgGs are glycoprotein antibodies that regulate immunological defense mechanisms such as phagocytosis and inflammatory responses . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibodies are specific for mouse IgG subclasses G1, G2a, G2b, and G3 and do not react with serum proteins in rat.
Immunogen
Mouse IgG
Application
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is suitable for use in flow cytometry, immunohistochemistry, indirect immunofluorescence, and immunohistochemistry .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with rat samples.
Other Notes
Antibody adsorbed with rat serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | sheep |
| Quality Segment | 200 |
| conjugate | FITC conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| technique(s) | direct immunofluorescence: 1:128 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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