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General description
L-amino-acid oxidase (UniProt: Q96RQ9; also known as EC:1.4.3.2, LAAO, LAO, Interleukin-4-induced protein 1, IL4-induced protein 1, Protein Fig-1, hFIG1) is encode by the IL4I1 (also known as FIG1, UNQ636/PRO1265) gene (Gene ID: 259307) in human. LAAO is a flavoenzyme containing, non-covalently bound flavin adenine dinucleotide enzyme. It is widely distributed and catalyzes the stereospecific oxidative deamination of L-amino acids to produce alpha-keto acids and it also produces ammonia and hydrogen peroxide via an imino acid intermediate. The initial step in its action involves removal of two hydrogen atoms by the flavin coenzyme, with formation of an unstable -amino acid intermediate, which undergoes decomposition by addition of water and forms the ammonium ion and the corresponding -keto acid. LAAO has been shown to selectively bind to cell membranes of specific phospholipid composition and deliver the hydrogen peroxide to localized regions on the cell surface thereby inducing local oxidative stress. Common substrates for LAAO include L-isomers of leucine, isoleucine, nor-leucine, alpha-amino butyric acid, phenylalanine, tyrosine, tryptophan norvaline, methionine, histidine, and citrulline. LAAO is believed to contain three hydrophobic substrate binding sites, designated a, b, and c. Subsite a accommodates one methylene carbon, subsite b two, and subsite c accommodates three carbons. It also contains an amino binding subsite, which is designated as the d site. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human L-Amino acid oxidase.
Application
Anti-L-amino acid oxidase, clone 4O11 ZooMAb, Cat. No. ZRB1022, is a recombinant Rabbit monoclonal antibody that targets L-Amino acid oxidase and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected L-amino acid oxidase in lysates from human colon tissue, THP-1 cells, rat spleen, and mouse spleen tissues.
Affinity Binding Assay: A representative lot of this antibody bound L-Amino Acid Oxidase peptide with a KD of 7.0 x 10-9 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: 1:100 from a representative lot detected L-amino acid oxidase in human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected L-amino acid oxidase in HeLa, A431, HepG2 and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 4O11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects L-Amino acid oxidase. It targets an epitope with 22 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4O11, recombinant monoclonal,monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 62.88 kDa,observed mol wt ~58 kDa |
| species reactivity | human, mouse, rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q96RQ9 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IL4I1(259307) |
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