Enterococcus faecium FISH probe – ATTO 488，50UL，MBD0047-50UL，Sigma

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells. Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples. FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood and tissue), microbial ecology (solid biofilms, aquatic systems) and plants. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest. Enterococcus faecium probe specifically recognizes E. faecium cells,. E. faecium a gram-positive bacterium and is a cause of a variety of infections, including endocarditis, urinary tract infections, prostatitis, intra-abdominal infection, cellulitis, and wound infection as well as concurrent bacteremia. Studies have shown that E. faecium is highly resistant to multiple antibiotics and that vancomycin-resistant Enterococcus faecium (VRE) can be asymptomatically carried by healthy people, which can hamper hospitals’ attempts to control the spread of the bacteria. Over the past two decades, Enterococcus faecium has emerged as a leading cause of multidrug-resistant enterococcal infection in the United States. E. faecium has a high antibiotic-resistance with more than half of its pathogenic isolates expressing resistance to vancomycin, ampicillin, and high-levels of aminoglycosides.

Application

Probe for fluorescence in situ hybridization (FISH), recognizes Enterococcus faecium cells

Features and Benefits

• Visualize, identify, and isolate E. faecium cells.
• Observe native E. faecium cell populations in diverse microbiome environments.
• Specific, sensitive, and robust identification of E. faecium in bacterial mixed population.
• Specific, sensitive, and robust identification even when E. faecium is in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on E. faecium morphology and allows to study biofilm architecture.
• Identify E. faecium in clinical samples such as tumor and brain tissues (for example in formalin-fixed paraffin-embedded (FFPE) samples), saliva and oral cavity and medical equipment such as dental implants and voice prostheses.
• The ability to detect E. faecium in its natural habitat is an essential tool for studying host-microbiome interaction.