Anti-DSC2 antibody produced in rabbit，100UL，HPA012615-100UL，Sigma

General description

Desmocollin 2 (DSC2) is a type I membrane glycoprotein that is localized in the cardiac tissue and other desmosome-containing tissues. It is expressed as two different splice variants with different carboxy-termini. DSC2a isoform possesses a larger cytoplasmic domain than the DSC2b isoform. The gene encoding this protein is present on chromosome 18. DSC2 contains four extracellular amino terminal domains, that is, an extracellular anchor domain (EA), a short transmembrane domain (TM), an intracellular anchor domain (IA), and an intracellular cadherin-binding domain (ICS). It has a single transmembrane domain and a cytoplasmic tail at the carboxy terminus.

Immunogen

Desmocollin-2 precursor recombinant protein epitope signature tag (PrEST)

Application

Anti-DSC2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige. Anti-DSC2 antibody produced in rabbit can also be used for immunoprecipitation.

Biochem/physiol Actions

Desmocollin 2 (DSC2) homo- and heterodimerize with each other and links the neighbouring cells by the interactions with the help of their extracellular cadherin domains. The domains also help these proteins to bind to desmogleins in a Ca²⁺-dependent manner. Its intracellular portions interact with plakoglobin (PG) and plakophilin-2 (PKP2). These two proteins in turn bind to desmoplakin (DSP) and provide a link to intermediate filaments. Mutations in the gene encoding DSC2 lead to arrhythmogenic right ventricular cardiomyopathy. DSC2 is essential for the establishment of early cardiac morphogenesis, normal myocardial structure and function and desmosome formation.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST72496

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.