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General description
UDP-glucose 6-dehydrogenase (UniProt: O60701; also known as EC:1.1.1.22, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH) is encoded by the UGDH gene (Gene ID: 7358) in human. UGDH is a key enzyme that catalyzes the conversion of UDP-glucose to UDO-glucuronic acid (UDP-GlcA) through the concomitant reduction of NAD+ to NADH. Its expression is observed in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine, and colon. It is required for the biosynthesis of chondroitin sulfate and heparan sulfate and is essential for embryonic development via its role in the biosynthesis of glycosaminoglycans. UGDH goes through several conformation states. In the initial state it is in an inactive conformation (E*) and substrate binding triggers a change to the active conformation (E). Its activity is inhibited by UDP-alpha-D-xylose that acts as a feedback inhibitor. It binds at the same site as the substrate, but functions as allosteric inhibitor by triggering a conformation change that disrupts the active hexameric ring structure and gives rise to an inactive, horseshoe-shaped hexamer. Mutations in UGDH gene are known to cause developmental and epileptic encephalopathy 84 (DEE84) that causes severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Three isoforms of UGDH have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hengel, H., et al. (2020). Nat. Commun. 11; Article number 595; Vigetti, D., et al. (2006). J. Biol. Chem. 281(12); 8254-8263).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
His-tagged recombinant fragment corresponding to 191 amino acids from the C-terminal region of human UDP-glucose 6-dehydrogenase (UDGH).
Application
Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UGDH in A549 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected UGDH in HeLa, HepG2, and COS-7 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected UGDH in A549 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected UGDH in human prostate tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant Human UGDH protein fragment with a KD of 1.5 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1F18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects UDP-glucose 6-dehydrogenase (UDGH). It targets an epitope within the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1F18, recombinant monoclonal |
| description | 1F18 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 55.02 kDa,observed mol wt ~55 kDa |
| purified by | using Protein A |
| species reactivity | human, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_003350 |
| UniProt accession no. | O60701 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... UDGH(7358) |
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