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General description
Rho-associated protein kinase 2 (UniProt: O75116; also known as EC:2.7.11.1, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2) is encoded by the ROCK2 (also known as KIAA0619) gene (Gene ID: 9475) in human. Rho kinase (ROCK), a serine/threonine kinase, serves as a target protein for small GTP-binding protein Rho and an important mediator of numerous cellular functions, including focal adhesions, motility, smooth muscle contraction, and cytokinesis. Rho kinase is found in two forms, ROCK 1 and ROCK2. They share 65% overall identity and 92% identity in the kinase domain. ROCK2 is mainly localized in the cytoplasm where it associates with actin microfilaments and the plasma membrane. It contains an amino-terminal catalytic kinase domain (aa 92-354) , a central coiled-coil domain of about 600 amino acids, and a carboxyl-terminal pleckstrin homology (PH) domain (aa 1150-1349) that is split by a cysteine-rich region. An interaction between its threonine 414 and aspartic acid 48 is shown to be essential for its kinase activity and dimerization. Phosphorylation at tyrosine 722 is reported to reduce its binding to RHOA and is crucial for focal adhesion dynamics. Since the ROCK-mediated pathway plays an important role in vascular smooth muscle contraction, cell adhesion and cell motility, it has gained importance in the pathogenesis of atherosclerosis. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hartmann, S., et al. (2015). Front. Pharmacol. 6; 276; Shi, J., et al. (2013). Cell Death Disease. 4; e483).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Rho-associated protein kinase 2 (ROCK2).
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ROCK2 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ROCK2 in A549, Raw264.7, and C6 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ROCK2 in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ROCK2 in C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound ROCK2 peptide with a KD of 1.8 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ROCK2 in HepG2 cell lysate.
Biochem/physiol Actions
Clone 1B20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Rho-associated protein kinase 2 (ROCK2). It targets an epitope within 21 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B20, recombinant monoclonal |
| description | 1B20 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 160.9 kDa,observed mol wt ~165 kDa |
| purified by | using Protein A |
| species reactivity | rat, human, mouse |
| species reactivity (predicted by homology) | monkey, canine, feline, horse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_001308572 |
| UniProt accession no. | O75116 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ROCK2(9475) |
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