蛋白质结晶冷冻试剂盒，1KIT，75403-1KT-F，Sigma

Application

The Crystallography Cryo Kit for Proteins provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.

Biochem/physiol Actions

Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization.

Features and Benefits

• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, flow charts, and observation sheets
• Low incidence of undesirable solubility effects

Other Notes

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.