- 商品介绍
- 规格参数
- 包装参数
General description
Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.
Application
The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.
Biochem/physiol Actions
Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase.
Physical form
Supplied as a lyophilized powder containing Tris-HCl and NaCl.
Analysis Note
Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).
Other Notes
One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.
| recombinant | expressed in E. coli BL21 |
| Quality Level | 200 |
| form | lyophilized solid |
| specific activity | ≥10 units/mg protein |
| mol wt | 26.0 kDa |
| shipped in | dry ice |
| storage temp. | −20°C |
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¥ 1573.49
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¥ 1779.22
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¥ 8982.64
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¥ 2277.64
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¥ 2132.12
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