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General description
S-phase kinase-associated protein 2 (UniProt: Q13309; also known as Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2) is encoded by the SKP2 (also known as FBXL1) gene (Gene ID: 6502) in human. Skp2 is the substrate recognition component of the SCF (Skp1 Cullin1 F-box (SCF) E3 ligase complex that mediates the ubiquitination and subsequent degradation of target proteins involved in cell cycle progression, signal transduction, and transcription. It plays an important role in governing cell cycle progression and cell survival by promoting the destruction of numerous tumor suppressor proteins, including p27, p21, p57, p130, and FOXO1. Hence, it functions as a proto-oncogene. It is shown to be essential for p27 degradation and thereby limits cells in G1 phase, prior to entry into S-phase. It specifically recognizes the phosphorylated form of p27. It also promotes ubiquitination and destruction of cadherin 1 (CDH1) in a CK1-dependent manner and hence regulates cell migration. Skp2 can undergo acetylation at lysine 68 and 71 that increases its stability through impairment of anaphase promoting complex/cyclosome (APC/C)-mediated proteolysis and promotes its cytoplasmic retention. Overexpression of Skp2 has been reported in a variety of human cancers, including lymphomas, prostate cancer, colorectal cancer, melanoma, pancreatic cancers, and nasopharyngeal carcinoma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Inuzuka, H., et al. (2012). Cell. 150(1):179-93; Wang, Z., et al. (2012). Front. Oncol. 1(57); 57; Cardozo, T., and Pagano, M. (2004). Nat. Rev. Mol. Cell Biol. 5(9); 739-751).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human S-phase kinase-associated protein 2 (Skp2).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Skp2 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Skp2 in U2OS, Cos-7, and PC3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Skp2 in human lung cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Skp2 in NIH 3T3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound Skp2 with a KD of 3.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1B13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects S-phase kinase-associated protein 2 (Skp2). It targets an epitope within 19 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B13, recombinant monoclonal |
| description | 1B13 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 47.76 kDa,observed mol wt ~48 kDa |
| purified by | using Protein A |
| species reactivity | monkey, mouse, human |
| species reactivity (predicted by homology) | bovine, porcine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal |
| Protein ID accession no. | NP_005974 |
| UniProt accession no. | Q13309 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SKP2(6502) |
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