- 商品介绍
- 规格参数
- 包装参数
General description
IL21 (interleukin 21) is a member of the γ-chain type I cytokine family, and a four-α-helix bundle pleiotropic cytokine. This gene is localized to human chromosome 4q26–q27. Although, it is predominantly produced by T follicular helper (TFH) and T helper-17 (TH17) cells, it is also produced by CD4+ T cells, neutrophils and natural killer cells. Under certain conditions, it is also produced by CD8+ T cells. Recombinant human IL-21 is a 15.0 kDa protein consisting of 132 amino acid residues.
Biochem/physiol Actions
IL21 (interleukin 21) functions on its heterodimeric receptor IL21R, which is expressed widely on immune and non-immune cells. Therefore, IL21 exerts its effects on a wide range of cell types and has a pleiotropic role in immune response regulation. This protein activates Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway and functions through mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)-AKT signaling pathways. Studies show that in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS), IL21 facilitates migration, invasion and production of MMPs (MMP-2, MMP-3, MMP-9, MMP-13) (matrix metalloproteinases). Therefore, it has potential as a therapeutic target in the treatment of RA. Studies show that CD3+TCRvβ11+ NK (natural killer) T cells isolated from a local Mycobacterium tuberculosis infection site produce IL21, which in turn assists B cells to produce and release IgG (immunoglobulin) and IgA, to help fight the infection.
Physical form
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | ≤0.5 ng/mL ED50 |
| mol wt | 15.4 kDa |
| packaging | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| UniProt accession no. | Q9HBE4 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IL21(59067) |
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¥ 4164.19
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¥ 1683.78
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
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¥ 2277.64
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¥ 30616.15
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