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General description
Monoclonal Anti- α Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. α-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, α-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, α-actinin has been detected in dense bodies and plaques characteristic of the tissue.
Immunogen
Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.
Application
Anti-α Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-α-Actinin may be used for immunofluorescent localization of α-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of α-actinin by immunoblotting analysis.
Biochem/physiol Actions
α-actinin has extensive proteins association with actin containing stress fibers, in particular with their membrane bound termini.
α-actinin modulates muscle contraction and has a role in cytoskeletal organisation. α-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.
Monoclonal Anti-α-Actinin specifically recognizes α-Actinin from human1, mouse2, rat3, chicken4, monkey, canine and bovine origin.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | BM-75.2, monoclonal |
| mol wt | ~100 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 0.5-1 μg/mL using human HeLa cells extract.,immunofluorescence: 5-10 μg/mL using human foreskin fibroblast Hs68 cells. |
| isotype | IgM |
| UniProt accession no. | P12814 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ACTN1(87) |
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