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General description
Ras-related protein Rab-7A (UniProt: P51149; also known as Rab-7a) is encoded by the RAB7A (also known as RAB7) gene (Gene ID: 7879) in human. Rab7A is a highly conserved member of the large superfamily of Ras-like GTPases. It is widely expressed, peripheral membrane protein that is localized mainly to late endosomes and regulates transport from early endosomes to late endosomes and from late endosomes to lysosomes. It is not only important for vesicle formation, transport and fusion but also for cargo selection and sorting. Like other GTPases, Rab7 also undergoes characteristic cyclical mechanism of activation and inactivation depending on GTP binding and hydrolysis. It also plays a many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transporter mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. It also participates in some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes and autophagosomes. More recently, it is reported to be essential for vesicular trafficking and cell surface expression of ACE2 and loss of Rab7A is reduces SARS-CoV-2 entry into cells by sequestering the ACE2 receptor inside cells through altered endosomal trafficking. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Daniloski, Z., et al. (2021). Cell. 184(1); 92-105; Guerra, F., and Bucci, C. (2016). Cells. 5(3); 34).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Rab7A.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Rab7A in human kidney tissue sections.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab7A in Cos-7 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Rab7A with a KD of 2.2 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Rab7A in HeLa, A431, HepG2 and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 4E15 is a Rabbit recombinant monoclonal antibody that specifically detects Rab7A. It targets an epitope within 17 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4E15, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 23.49 kDa,observed mol wt ~30 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_004628.4? |
| UniProt accession no. | P51149 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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