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General description
Podocalyxin (UniProt: O00592; also known as GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1) is encoded by the PODXL (also known as PCLP, PCLP1) gene (Gene ID: 5420) in human. Podocalyxin is a single-pass type I membrane glycoprotein, which is synthesized with a signal peptide (aa 1-22) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-461), a transmembrane domain (aa 462-482), and a cytoplasmic domain (aa 483-558). Its expression is observed in glomerular epithelium cells (podocytes). It exists in a monomeric form when associated with the membrane raft, but oligomerizes when integrated in the apical membrane. Podocalyxin functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. It can also act as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It is reported to induce the formation of apical actin-dependent microvilli and is involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. It is reported to play a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. It affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Two isoforms of Podocalyxin have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nielsen, JS., and McNagny, KM (2009). J. Am. Soc. Nephrol. 20(8); 1669-1676; Sizemore, S., et al. (2007). Cancer Res. 67(13); 6183-6191).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human Podocalyxin.
Application
Quality Control Testing
Evaluated by Western Blotting in Human kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocalyxin in Human kidney tissue lysate.
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected Podocalyxin in one million NTERA-2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Podocalyxin peptide with a KD of 1.3 x 10-6 in an affinity binding assay.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Podocalyxin in Human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Podocalyxin in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1E13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Podocalyxin (PODXL). It targets an epitope within 25 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1E13, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.64 kDa,observed mol wt ~170 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001018121 |
| UniProt accession no. | O00592 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... PODXL(5420) |
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¥ 1148.92
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