- 商品介绍
- 规格参数
- 包装参数
General description
GenElute HP Endotoxin-Free Plasmid Megaprep Kit 可以简单、快速、低成本的为从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。在1.5小时内可从200 mL - 1.0L LB培养基或200 - 600 mL大肠杆菌TB培养基纯化出多达5 mg内毒素水平≤ 0.1 EU/μg高拷贝数质粒DNA。请注意,实际得率取决于所用的菌株、质粒和培养基。
该产品可为包含内毒素敏感细胞的限制性酶切、连接、测序、PCR、转化和转染等下游应用提供优质、不含内毒素的DNA。
Click here to view the kit protocol
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.
该产品获得高质量、不含内毒素的DNA,得到的DNA可进行诸多下游应用,如限制性酶切、连接、测序、PCR、转化和内毒素敏感细胞的转染等。
Features and Benefits
- 可纯化出多达5 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒,仅需90分钟
- 采用真空处理形式,无需进行乙醇沉淀
Other Notes
更多信息,请见www.sigma-aldrich.com/genelutehp。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
| Quality Segment | 200 |
| usage | kit sufficient for 5 preparations |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry. |
| sustainability | Greener Alternative Product |
| test parameters | sample volume: 200-1000 mL bacterial culture |
| greener alternative category | , Aligned |
| storage temp. | 15-25°C |
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