Anti-IDH1 Antibody, clone 5S2U7, Rabbit Monoclonal，100UL，SAB5702871-100UL，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IDH1 (NP_005887.2).
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

Application

WB, IHC, IF/ICC, IP

技术参数 Specifications

biological source	rabbit
Quality Segment	100
conjugate	unconjugated
material	colorless
clone	5S2U7, monoclonal
form	liquid
mol wt	45 kDa
species reactivity	rat, human
concentration	1.36 mg/mL
technique(s)	immunofluorescence: 1:50 - 1:200,immunohistochemistry: 1:50 - 1:200,immunoprecipitation (IP): 1:50 - 1:200,western blot: 1:500 - 1:2000
color	colorless
isotype	IgG
immunogen sequence	DATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG
UniProt accession no.	O75874
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified
Gene Information	human ... IDH1(3417)

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