- 商品介绍
- 规格参数
- 包装参数
General description
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Immunogen
Human CD34 positive bone marrow cells
Biochem/physiol Actions
The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
Physical form
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | phycoerythrin (R-PE) conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | STRO-1, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human, ( Rabbit), ( Rat) |
| concentration | 0.1 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgMλ |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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