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General description
G0/G1 switch protein 2 (UniProt: P27469; also known as G0/G1 switch regulatory protein 2, Putative lymphocyte G0/G1 switch gene, G0S2) is encoded by the G0S2 gene (Gene ID: 50486) in human. G0S2 is a highly conserved, mitochondrial protein that is widely expressed in vertebrate species. High expression of G0S2 is reported in peripheral blood cells, skeletal muscle, heart, liver and kidney. It is multifaceted protein that is involved in cell proliferation, apoptosis, inflammation, metabolism, and carcinogenesis. The G0S2 gene is reported to be hypermethylated in several human cancer cell lines. Its promoter region contains potential binding sites for AP1, AP2, AP3, and Nuclear factor of activated T cells (NFAT). The G0S2 protein is shown to contain two -helices that are separated by a hydrophobic sequence with the potential to generate turns and assume a β-sheet conformation. It also has several putative phosphorylation sites for protein kinase C (PKC) and casein kinase II. G0S2 can interact with Bcl2 and block the formation of Bcl2-Bax heterodimer to block apoptosis. In fat tissue it acts as a molecular brake on triglyceride catabolism. G0S2 is reported to be a major selective inhibitor of adipose triglyceride lipase (AGTL) and its hydrolase activity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Heckmann, BL., et al. (2013). Biochim. Biophys. Acta. 1831(2); 276-281; Heckmann, BL., et al. (2016). PLoS ONE 11(6); e0156742).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Immunogen
GST/His-tagged full-length recombinant human G0/G1 switch protein 2 (G0S2).
Application
Quality Control Testing
Evaluated by Western Blotting in Rat Heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected G0S2 in Rat Heart tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected G0S2 in lysates from human heart, mouse heart, and A7R5 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected G0S2 in rat heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound G0S2 protein with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-G0S2, clone 2C13 ZooMAb®, Cat. No. ZRB1813, is a recombinant Rabbit monoclonal antibody that specifically targets G0S2 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Biochem/physiol Actions
Clone 2C13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects G0/G1 switch protein 2 (G0S2).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C13, monoclonal,recombinant monoclonal |
| description | 2C13 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt ~11.32 kDa,observed mol wt ~8 kDa |
| species reactivity | rat, mouse, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P27469 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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