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General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Integrin a6 (UniProt: P23229; also known as CD49 antigen-like family member F, VLA-6, CD49f) is encoded by the ITGA6 gene (Gene ID: 3655) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrin a6 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-1050), a transmembrane domain (aa 1051-1076), and a cytoplasmic domain (aa 1077-1130). It can heterodimerize with b1 to form a receptor for laminin on platelets. a6/b1 is also present in oocytes and is involved in sperm-egg fusion. Integrin a6/b4 heterodimer is a receptor for laminin in epithelial cells and plays a role in the hemidesmosome. It also binds to IGF-1 and IGF-2 and these binding are shown to be essential for IGF signaling. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal, extracellular domain of human Integrin a6.
Application
Quality Control Testing
Evaluated by Western Blotting in Cos-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Integrin a6/CD49f in Cos-7 cell lysate.
Tested applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Integrin a6/CD49f in HeLa, A431, HUVEC, and NIH3T3 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Integrin a6/CD49f in one million human peripheral blood mononuclear cells (PBMC).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin a6/CD49f in human stomach tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Integrin a6/CD49f, clone 5I3 ZooMAb®, Cat. No. ZRB1168, is a recombinant Rabbit monoclonal antibody that targets Integrin a6 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Biochem/physiol Actions
Clone 5I3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Integrin a6. It targets an epitope within 17 amino acids from the C-terminal half, extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 5I3, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 126.61 kDa,observed mol wt ~130 kDa |
| purified by | using Protein A |
| species reactivity | monkey, human, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_000201.2? |
| UniProt accession no. | P23229 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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