Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse，10UG，S5816-10UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

Application

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

Biochem/physiol Actions

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.
SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Preparation Note

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Analysis Note

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

技术参数 Specifications

biological source	mouse
Quality Segment	200
recombinant	expressed in E. coli
assay	>97% (SDS-PAGE)
form	powder
potency	0.15-0.6 ng/mL ED₅₀
quality	endotoxin tested
mol wt	predicted mol wt ~8 kDa
packaging	pkg of 10 μg
storage condition	avoid repeated freeze/thaw cycles
technique(s)	cell culture \| mammalian: suitable
impurities	<1 EU/μgtested (LAL test)
UniProt accession no.	P40224
storage temp.	−20°C

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