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General description
Gap junction delta-2 protein (UniProt: Q9UKL4; also known as Connexin-36, Cx36, Gap junction alpha-9 protein) is encoded by the GJD2 (also known as GJA9) gene (Gene ID: 57369) in human. Cx36 is a multi-pass membrane protein with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. It is mainly expressed in neurons where it forms electrical synapses and in pancreatic β cells where it plays an important role in insulin secretion and glycemic control. It is regulated by intracellular pH and cytosolic magnesium ions, which can vary significantly under various physiological and pathological conditions. Its levels are upregulated under conditions of low intracellular pH and Mg2+. It exhibits a very low unitary conductance and low sensitivity to transjunctional voltage. Cx36 knockout (Cx36 -/-) mice exhibit impaired visual transmission and elevated auditory brainstem response threshold. Deletion of Cx36 in mice is shown to cause the loss of functional synapses between GABAergic interneurons in hippocampal slices. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rimkute, L., et al. (2018). Front. Physiol. 9; 362).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal, cytoplasmic domain of human Connexin-36.
Application
Quality Control Testing
Evaluated by Western Blotting in PANC-1 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Connexin-36 in PANC-1 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Connexin-36 in lysates from Rat brain tissue, Human brain tissue and recombinant Bovine Connexin-36.
Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected Connexin-36 peptide in ELISA application.
Affinity Binding Assay: A representative lot of this antibody bound Connexin-36 peptide with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 2D22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Connexin 36. It targets an epitope within 17 amino acids from the C-terminal, cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2D22, recombinant monoclonal |
| description | 2D22 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 36.09 kDa,observed mol wt ~38 kDa |
| purified by | using Protein A |
| species reactivity | bovine, human, rat |
| species reactivity (predicted by homology) | feline, porcine, monkey, mouse, canine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_065711.1 |
| UniProt accession no. | Q9UKL4 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... GJD2(57369) |
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