Mouse CRISPR Brie Knockout Library，1EACH，PCRISPR004-1EA，Sigma

General description

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Application

• Functional Genomics/Target Validation
• Unbiased wholed genome forward genetic screening
• Validated positive and negative controls
• Set up and optimization of screen assay

Biochem/physiol Actions

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

Features and Benefits

• Focus on your research, and we will generate your lentivirus screening library.
• Use CRISPR nucleases to knockout protein-coding genes to assess their function.
• Mouse Genes targeted 19,674
• Compact library of ~ four gRNAs per gene (78,637 total)
• Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.

Legal Information

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES