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- 规格参数
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Application
Protein L-agarose is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, phosphorylation analysis, and protein A, G and L resins. Protein L-agarose has been used to provide evidence that antineuronal antibodies may contribute to neuronal dysfunction observed in a subset of patients with neurogenic chronic intestinal pseudoobstruction. Protein L agarose has also been used to evaluate a diabody to improve protection againse a potent scorpion neurotoxin.
Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding site. Recombinant Protein L contains four Ig-binding domains.
Preparation Note
Prepared with recombinant Peptostreptococcus magnus Protein L.
| recombinant | expressed in E. coli |
| Quality Segment | 200 |
| matrix | 6% beaded agarose supplied as 50% slurry |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| capacity | 3-10 mg/mL binding capacity |
| storage temp. | 2-8°C |
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