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General description
Aryl hydrocarbon receptor nuclear translocator (UniProt: P27540; also known as ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta) is encoded by the ARNT (also known as BHLHE2) gene (Gene ID: 405) in human. ARNT is a member of the basic Helix-Loop-Helix PER/ARNT/SIM (bHLH/PAS) protein family and is conserved as an important transcriptional regulator in organogenesis, neural development, and physiological adaptation to hypoxia. It exhibits about 90% amino acid identity with ARNT2, but is more ubiquitous in its distribution in adult tissues. It exists as a monomer, but dimerizes with Aryl Hydrocarbon Receptor (AhR) to form a heterodimer that binds to core DNA sequence 5′-TGCGTG-3′ within the dioxin response element (DRE) of target gene promoters and activates their transcription. ARNT also forms a heterodimer with HIF-1a and that dimer promotes cell survival and angiogenesis at varying oxygen levels. It facilitates normoxia under adverse conditions. ARNT contains one bHLH domain (aa 89-142), two PAS (Per-Arnt-Sim) domains (aa 161-235 and 349-419) and one PAC domain (aa 424-467). Despite the presence of a nuclear export sequence, it mainly accumulates in the nucleus. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wolff, M., et al (2013). Cell Physiol. Biochem. 32(4); 849-858
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Aryl hydrocarbon receptor nuclear translocator (ARNT).
Application
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARNT in NIH3T3 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ARNT in L6 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ARNT in MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ARNT in human uterus tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ARNT with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1H2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Aryl hydrocarbon receptor nuclear translocator (ARNT). It targets an epitope within 17 amino acids from the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H2, recombinant monoclonal |
| description | 1H2 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 86.64 kDa,observed mol wt ~87 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | canine, monkey, feline, porcine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_001659 |
| UniProt accession no. | P27540 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ARNT(405) |
| 长度(mm) | |
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