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General description
Ras-related protein Rab-14 (UniProt: P61106; also known as Rab14) is encoded by the RAB14 gene (Gene ID: 51552) in human. Rab proteins constitute a large family of the low-molecular-weight GTPases that play key roles in the regulation of intracellular membrane trafficking. They also play a role in cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. They act as molecular switches that flip between a GDP-bound inactive state and a GTP-bound active form in which they recruit downstream effector proteins onto membranes. Rab14 is involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. It is reported to regulate the Golgi to endosome transport of FGFR-containing vesicles during early development. It may also act by modulating the kinesin KIF16B-cargo association to endosomes. It is also involved in phagosome and early endosome fusion. It is shown to be essential for the formation of PtdIns(4,5)P2-enriched lipid domains that promote the recruitment of downstream effectors of polarity, including Par3, aPKC and Cdc42. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Linford, A., et al. (2012). Dev. Cell. 22(5); 952-966; Junutula, JR., et al. (2004). Mol. Biol. Cell. 15(5); 2218-2229).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated liner peptide corresponding to 23 amino acids from the C-terminal region of human Ras-related protein Rab-14.
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Rab14 in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Rab14 in lysate from Mouse brain tissue and 1:1,000 dilution detected Rab14 in Rat brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Rab14 in Human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Rab14 peptide with a KD of 8.4 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rab14 in U-251 MG cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1M15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Ras-related protein Rab-14. It targets an epitope within 23 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | Clone, 1M15 |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 24 kDa,observed mol wt ~24 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human, rat |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_057406 |
| UniProt accession no. | P61106 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... RAB14(51552) |
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