CRISPR & MISSION® Lentiviral Packaging Mix,0.25ML,SHP002-0.25ML,Sigma

销售价: ¥ 4824.55 / 件
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订货号 0BT6030
品牌型号 Sigma SHP002-0.25ML
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Lentiviral particles are generated from three main components:

  • The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
  • The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
  • The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.


Application

Functional Genomics/Screening/Target Validation


Legal Information

CRISPR Use License Agreement
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany


技术参数 Specifications
concentration100 ng/μL (in Tris-EDTA)
Quality Level200
application(s)CRISPR
shipped indry ice
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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