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General description
E-selectin (UniProt: P16581; also known as CD62E, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1 (ELAM-1), Leukocyte-endothelial cell adhesion molecule 2 (LECAM2)) is encoded by the SELE (also known as ELAM1) gene (Gene ID: 6401) in human. E-selectin is single-pass type I membrane glycoprotein, a member of the selectin family of adhesion molecules, characterized by an extracellular domain containing a C-type lectin domain (aa 22-139), an EGF-like domain (aa 140-175), and a short cytoplasmic tail. E-selectin plays a crucial role in the immune response by mediating the adhesion of leukocytes to endothelial cells during inflammation. Upon activation by pro-inflammatory cytokines (such as TNF- and IL-1), E-selectin is expressed on the surface of endothelial cells, allowing leukocytes to roll along the vessel wall and eventually migrate into tissues. It is not constitutively expressed but is upregulated during inflammatory processes, such as in response to cytokines. E-selectin expression can vary depending on tissue type and the presence of inflammatory signals. E-selectin is localized on the surface of endothelial cells. It is stored in Weibel-Palade bodies, which are specialized storage granules within endothelial cells, and is translocated to the cell surface upon activation by inflammatory mediators. E-selectin is implicated in various inflammatory and cardiovascular diseases. Elevated levels of E-selectin are associated with chronic inflammatory conditions, such as atherosclerosis, rheumatoid arthritis, and asthma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human CD62E.
Application
Quality Control Testing
Evaluated by Western Blotting in human bone marrow tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD62E in lysate from human bone marrow tissue.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD62E in human bone marrow tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound with CD62E peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1I3-H1 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CD62E. It targets an epitope within 17 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1I3-H1, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 66.66 kDa,observed mol wt ~62 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | rat |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| concentration | 0.3 mg/mL |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_000441 |
| UniProt accession no. | P16581 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... SELE(6401) |
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