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General description
Sirtuin 3 (SIRT3) is a member of a family of NAD+-dependent protein deacetylases called sirtuins (SIRTs). In mammals, there are seven types of SIRTs (SIRT1 to 7). SIRT 3 is present in mitochondrial matrix.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Sirtuin 3 (SIRT3) is thought to be involved in metabolism as its expression is suppressed in muscles of diabetic animals and increased in white and brown adipose tissues during calorie restriction (CR). This protein modulates the degree of acetylation and activity of acetyl-coenzyme A synthetase 2, an enzyme thought to be involved in energy production during starvation conditions in mammals. Up-regulation of this gene influences the expression of genes involved in mitochondrial function. During fasting, SIRT3 controls mitochondrial intermediary metabolism and fatty-acid use by deacetylating long-chain acyl coenzyme A dehydrogenase (LCAD). It is an essential modulator of basal ATP, and therefore, overall energy homeostasis.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 59 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_012239 |
| UniProt accession no. | Q9NTG7 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SIRT3(23410) |
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| 重量(kg) |
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