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General description
Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physicochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies.
Monoclonal Anti-Human IgG3 (mouse IgG1 isotype) is derived from the hybridoma (HP-60501) produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
Purified myeloma human IgG3 coupled to polyaminostyrene (PAS) microbeads was used as the immunogen
Application
The product may be used for the identification of human IgG3 subclass by means of various immunoassays and ELISA. It has also been used in immunoblotting.
Biochem/physiol Actions
该抗体对人 IgG3 具有特异性，与其他 IgG 亚类不反应。IUIS/WHO 研究推荐该克隆在广泛的检测方案和技术中具有特异性和反应性。
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG3 antibody allows for the binding of various labels such as avidin or streptavidin.
Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. IgG3 has O-glycosylation sites and mediates, phagocytosis, antibody-mediated cellular cytotoxicity, neutralization, and activates complement pathways. IgG3 subtypes have short in vivo half-life and mediates the effector functions than IgG1.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | biotin conjugate |
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| clone | HP-6050, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | direct ELISA: 1:40,000 |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
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¥ 2013.69
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¥ 1779.22
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无菌，PETG 方形有刻度培养基瓶，带盖，聚对苯二酸乙二醇酯共聚物，天然高密度聚乙烯盖，500毫升容量，每箱40，40/箱，342020-0500，Nalgene，Thermofisher，赛默飞世尔¥ 3684.34
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